Clostridium perfringens- Laboratory Diagnosis, Treatment, Prevention

Clostridium perfringens- Laboratory Diagnosis, Treatment, Prevention

Laboratory Diagnosis of Clostridium perfringens For Diarrheal Disease Laboratories diagnose C. perfringens food poisoning by detecting a type of bacterial toxin in feces or by tests to determine the number of bacteria in the feces. A count of at least 106 C. perfringens spores per gram of stool within 48 hours of when the illness … Read moreClostridium perfringens- Laboratory Diagnosis, Treatment, Prevention

Clostridium perfringens- Pathogenesis, Clinical Features

Clostridium perfringens- Pathogenesis, Clinical Features

Clostridium perfringens- Pathogenesis, Clinical Features Clostridium perfringens (previously named Clostridium welchii) is a Gram-positive, rod-shaped, anaerobic, spore-forming pathogenic bacterium, which is found commonly in decaying vegetation and soils.  Along with many environmental sources, it is also found in the intestines of humans and animals.  Clostridium perfringens is the causative agent of a number of human diseases, … Read moreClostridium perfringens- Pathogenesis, Clinical Features

Biochemical Test of Clostridium tetani

Biochemical Test of Clostridium tetani

Biochemical Test of Clostridium tetani Some of the characteristics are as follows: Basic Characteristics Properties (Clostridium tetani) Capsule Negative (-ve) Catalase Negative (-ve) Flagella Peritrichous Gas Positive (+ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Gram-stain-positive, but become Gram-stain-negative after approximately 24 h incubation. Growth in 20% Bile Negative (-ve) Growth in 6.5% NaCl Negative (-ve) … Read moreBiochemical Test of Clostridium tetani

Biochemical Test of Clostridium botulinum

Biochemical Test of Clostridium botulinum

Biochemical Test of Clostridium botulinum (Type A and proteolytic strains of types B and F) Basic Characteristics Properties (Clostridium botulinum) Ammonia Production Positive (+ve) 20% Bile Negative (-ve) Capsule Negative (-ve) Catalase Negative (-ve) Flagella Peritrichous Gelatin Hydrolysis Positive (+ve) Gram Staining Gram-positive H2S Positive (+ve) Hemolysis Positive (+ve). β-hemolytic Indole Negative (-ve) Milk Digestion … Read moreBiochemical Test of Clostridium botulinum

Biochemical Test of Clostridium perfringens

Biochemical Test of Clostridium perfringens

Biochemical Test of Clostridium perfringens Basic Characteristics Properties (Clostridium perfringens) 20% Bile Positive (+ve) Capsule Positive (+ve) Catalase Negative (-ve) Flagella Negative (-ve) Gas Positive (+ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Gram-positive Hemolysis Positive (+ve) Indole Negative (-ve) Motility Negative (-ve) Nitrate Reduction Variable Oxidase Negative (-ve) Shape Rod shaped; straight rods with blunt … Read moreBiochemical Test of Clostridium perfringens

Biochemical Test of Clostridium difficile

Biochemical Test of Clostridium difficile

Biochemical Test of Clostridium difficile ­Basic Characteristics Properties (Clostridium difficile) Capsule Capsulated Catalase Negative (-ve) Flagella Flagellated Gelatin Hydrolysis Positive (+ve) Gram Staining Positive (+ve) H2S Positive (+ve) Hemolysis Negative (-ve) Indole Negative (-ve) Motility Motile Nitrate Reduction Negative (-ve) OF (Oxidative-Fermentative) Anaerobic Oxidase Negative (-ve) Shape Rods Spore Positive (+ve) Urease Negative (-ve) VP … Read moreBiochemical Test of Clostridium difficile

Cultural characteristics of Clostridium perfringens

Cultural characteristics of Clostridium perfringens

Cultural characteristics of Clostridium perfringens It is an anaerobic bacteria but can grow under micro-aerophilic condition. They form large, translucent, flat and filamentous colonies with irregular edges. PH: 5.5 to 8.0 (Average PH: 7.2) Temperature: 20°C to 50°C (Average: 37°C) At 45°C, generation time is 10 minutes. Clostridium perfringens on Robertson’s Cooked Meat Broth Saccharolytic … Read moreCultural characteristics of Clostridium perfringens

Habitat of Clostridium perfringens

Habitat of Clostridium perfringens

Habitat of Clostridium perfringens Clostridium perfringens are isolated from fecal specimens from human and animals. They are an inhabitant of human normal intestinal flora. They are present in nature and can be found as a normal component of decaying vegetation, marine sediments and soil. Beef, poultry, gravies and dried or pre-cooked foods are common sources … Read moreHabitat of Clostridium perfringens

Morphology of Clostridium perfringens

Morphology of Clostridium perfringens

Morphology of Clostridium perfringens Clostridium perfringens is large rectangular gram positive bacilli with rounded or truncated ends. It is pleomorphic with straight or curved rods. Size is about 3-8 µm X 0.4-1.2 µm. It is capsulated, non-motile and non-flagellated pathogenic bacteria. It contains spores with central or sub-terminal spores but spores are rarely seen. Endospores … Read moreMorphology of Clostridium perfringens