Biochemical Test of Bacteria

Biochemical Test of Bacteria

Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are necessary for the bacterium’s individual metabolism, and some facilitate the bacterium’s ability to compete with other bacteria or establish an infection. Tests that measure single bacterial enzymes are simple, … Read more

Biochemical Test of Clostridium tetani

Biochemical Test of Clostridium tetani

Some of the characteristics are as follows: Basic Characteristics Properties (Clostridium tetani) Capsule Negative (-ve) Catalase Negative (-ve) Flagella Peritrichous Gas Positive (+ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Gram-stain-positive, but become Gram-stain-negative after approximately 24 h incubation. Growth in 20% Bile Negative (-ve) Growth in 6.5% NaCl Negative (-ve) H2S Positive (+ve) Hemolysis Positive (+ve) Indole Variable Motility Positive (+ve) Nitrate Reduction Negative (-ve) Shape Large rods (0.5-1.7 x 2.1-18.1 µm), which may appear single, in pairs or in … Read more