Laboratory Diagnosis of Clostridium perfringens For Diarrheal Disease Laboratories diagnose C. perfringens food poisoning by detecting a type of bacterial toxin in feces or by tests to determine the number of bacteria in the feces. A count of at least 106 C. perfringens spores per gram of stool within 48 hours of when the illness … Read more Clostridium perfringens- Laboratory Diagnosis, Treatment, Prevention
Clostridium perfringens- Pathogenesis, Clinical Features Clostridium perfringens (previously named Clostridium welchii) is a Gram-positive, rod-shaped, anaerobic, spore-forming pathogenic bacterium, which is found commonly in decaying vegetation and soils. Along with many environmental sources, it is also found in the intestines of humans and animals. Clostridium perfringens is the causative agent of a number of human diseases, … Read more Clostridium perfringens- Pathogenesis, Clinical Features
Biochemical Test of Clostridium perfringens Basic Characteristics Properties (Clostridium perfringens) 20% Bile Positive (+ve) Capsule Positive (+ve) Catalase Negative (-ve) Flagella Negative (-ve) Gas Positive (+ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Gram-positive Hemolysis Positive (+ve) Indole Negative (-ve) Motility Negative (-ve) Nitrate Reduction Variable Oxidase Negative (-ve) Shape Rod shaped; straight rods with blunt … Read more Biochemical Test of Clostridium perfringens
Objective of Lecithinase Test The objective is to determine the ability of microorganisms to produce the enzyme lecithinase and to identify the bacteria which are capable of producing lecithinase enzyme. Principle of Lecithinase Test Lecithinases or phospholipases are enzymes released by bacteria that have the ability to destroy animal tissues and play a role in pathogenecity. Lecithinase, which … Read more Lecithinase Test- Objectives, Principle, Procedure and Results
Cultural characteristics of Clostridium perfringens It is an anaerobic bacteria but can grow under micro-aerophilic condition. They form large, translucent, flat and filamentous colonies with irregular edges. PH: 5.5 to 8.0 (Average PH: 7.2) Temperature: 20°C to 50°C (Average: 37°C) At 45°C, generation time is 10 minutes. Clostridium perfringens on Robertson’s Cooked Meat Broth Saccharolytic … Read more Cultural characteristics of Clostridium perfringens
Habitat of Clostridium perfringens Clostridium perfringens are isolated from fecal specimens from human and animals. They are an inhabitant of human normal intestinal flora. They are present in nature and can be found as a normal component of decaying vegetation, marine sediments and soil. Beef, poultry, gravies and dried or pre-cooked foods are common sources … Read more Habitat of Clostridium perfringens
Morphology of Clostridium perfringens Clostridium perfringens is large rectangular gram positive bacilli with rounded or truncated ends. It is pleomorphic with straight or curved rods. Size is about 3-8 µm X 0.4-1.2 µm. It is capsulated, non-motile and non-flagellated pathogenic bacteria. It contains spores with central or sub-terminal spores but spores are rarely seen. Endospores … Read more Morphology of Clostridium perfringens