Campylobacter Blood Agar (CVA)- Composition, Principle, Preparation, Results, Uses

Campylobacter Blood Agar (CVA)

Campylobacter Blood Agar (CVA) is a selective medium used for the primary isolation of Campylobacter jejuni from stool specimens. Dekeyser et al. reported the isolation of Campylobacter jejuni from the feces of patients with diarrhea and acute gastroenteritis by using a filtration technique and a selective medium with antimicrobials to suppress the normal enteric flora whereas, in 1977, Skirrow reported a selective medium containing three antimicrobics for the isolation. In 1978, Blaser et al reported success in the isolation of C. jejuni … Read more

Biochemical Test of Bacteria

Biochemical Test of Bacteria

Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are necessary for the bacterium’s individual metabolism, and some facilitate the bacterium’s ability to compete with other bacteria or establish an infection. Tests that measure single bacterial enzymes are simple, … Read more

Biochemical Test of Campylobacter jejuni

Biochemical Test of Campylobacter jejuni

Biochemical Test of Campylobacter jejuni Basic Characteristics Properties (Campylobacter jejuni) Capsule Capsulated Catalase Positive (+ve) Flagella Flagellated Gram Staining Negative (-ve) Growth at 25°C Negative (-ve) Growth at 37°C Positive (+ve) Growth at 42°C Positive (+ve) Growth on MacConkey Agar Positive (+ve) H2S Negative (-ve) Hemolysis Non-Hemolytic KOH Positive (+ve) Motility Motile Nitrate Reduction Positive (+ve) Nitrite Reduction Negative (-ve) OF (Oxidative-Fermentative) Non-Fermentative Oxidase Positive (+ve) Resistance to cephalothin Positive (+ve) Resistance to nalidixic acid Negative (-ve) Shape Comma/S-Shaped/Coccoid/Rods Spore … Read more