Southern Blot- Principle, Steps and Applications

Southern Blot- Principle, Steps and Applications

The analytical technique that involves the transfer of a specific DNA, RNA or a protein separated on gel to a carrier membrane, for their detection or identification is termed as blotting. The process of transfer of the denatured fragments out of the gel and onto a carrier membrane makes it accessible for analysis using a … Read more Southern Blot- Principle, Steps and Applications

Western blotting- Introduction, Principle and Applications

Western blotting- Introduction, Principle and Applications

Introduction Western blot is the analytical technique used in molecular biology, immunogenetics and other molecular biology to detect specific proteins in a sample of tissue homogenate or extract. Western blotting is called so as the procedure is similar to Southern blotting. While Southern blotting is done to detect DNA, Western blotting is done for the … Read more Western blotting- Introduction, Principle and Applications