Chocolate Agar

Chocolate Agar

Chocolate agar is essentially the same as blood agar except that during preparation the red blood cells are lysed when added to molten agar base. As a result, the cell lysis releases intracellular nutrients such as hemoglobin, hemin (“X” factor), … Read more

Campylobacter Blood Agar (CVA)

Campylobacter Blood Agar (CVA)

Campylobacter Blood Agar (CVA) is a selective medium used for the primary isolation of Campylobacter jejuni from stool specimens. Dekeyser et al. reported the isolation of Campylobacter jejuni from the feces of patients with diarrhea and acute gastroenteritis by using a filtration … Read more

Columbia Agar with 5% Sheep Blood

Columbia Agar with 5% Sheep Blood

Columbia Agar with 5% Sheep blood is highly nutritious general purpose medium for the isolation and cultivation of the non-fastidious and fastidious microorganisms from various clinical specimens and non-clinical specimens of public health importance.  Columbia Blood Agar was first described … Read more

Brain Heart Infusion (BHI) Agar

Brain Heart Infusion (BHI) Agar

In 1919, Rosenow devised an excellent medium for culturing streptococci by using a dextrose broth supplemented with brain tissue. Rosenow’s formulation was later modified by Hayden who found the addition of crushed marble resulted in favorable growth of dental pathogens. … Read more

Anaerobic Blood Agar

Anaerobic Blood Agar

Anaerobic Blood Agar is a solid media recommended for use in qualitative procedures for primary isolation and cultivation of anaerobic organisms, including fastidious strains. This medium was formulated by V.R. Dowell and T.M. Hawkins at the Centers for Disease Control … Read more