Ashdown’s Agar- Composition, Principle, Preparation, Results, Uses

Ashdown's Agar

Ashdown’s medium is a selective culture medium for the isolation and characterization of Burkholderia pseudomallei (the bacterium that causes melioidosis). Ashdown’s medium was first described by LR Ashdown in 1979. Composition of Ashdown’s Agar Ingredients Gram/Litre Tryptone soya broth 10 g Agar                            15 g Glycerol 40 ml Gentamicin 4 mg Crystal violet 0.1% 5 ml Neutral Red 1% 5 ml Distilled water              950ml Principle of Ashdown’s Agar The medium contains crystal violet and gentamicin as selective agents to suppress the growth of other bacteria. Colonies of  B. pseudomallei … Read more