Coomb’s Test- Direct and Indirect Coomb’s Test

Result Interpretation of Coomb's Test

Coomb’s test is a direct agglutination reaction, more commonly known as antiglobulin test. It was discovered by Coombs, Mourant and Race in 1945 originally for the detection of incomplete anti-Rh antibodies. In the test, incomplete antibodies do not agglutinate erythrocytes. Incomplete antibody antiglobulin coats the surface of erythrocytes but does not cause any agglutination. When … Read moreCoomb’s Test- Direct and Indirect Coomb’s Test

Types of Agglutination Reactions

Antiglobulin (Coombs) test

Types of Agglutination Reactions Agglutination reactions can be broadly divided into three groups: Active/Direct agglutination Passive agglutination Hemagglutination 1. Active agglutination Agglutination reactions where the antigens are found naturally on a particle are known as direct agglutination. In active agglutination, direct agglutination of particulate antigen with specific antibody occurs. Direct bacterial agglutination uses whole pathogens … Read moreTypes of Agglutination Reactions

Agglutination- Introduction and Applications

Agglutination- Introduction and Applications

Agglutination- Introduction and Applications Agglutination is an antigen–antibody reaction in which a particulate antigen combines with its antibody in the presence of electrolytes at a specified temperature and pH resulting in formation of visible clumping of particles. Agglutination occurs optimally when antigens and antibodies react in equivalent proportions. This reaction is analogous to the precipitation … Read moreAgglutination- Introduction and Applications