Indirect ELISA- Introduction, Steps, Advantages and Protocol

Indirect ELISA- Introduction, Steps, Advantages and Protocol

Introduction Indirect ELISA is a two-step ELISA which involves two binding process of primary antibody and labeled secondary antibody. The primary antibody is incubated with the antigen followed by the incubation with the secondary antibody. However, this may lead to nonspecific signals because of cross-reaction that the secondary antibody may cause. In the indirect ELISA test, the … Read more Indirect ELISA- Introduction, Steps, Advantages and Protocol