Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation Objective of Kinyoun-Cold Method Identification of acid-fast Mycobacterium spp. and parasites such as Cryptosporidium and Isopora spp. Principle of Kinyoun-Cold Method Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells waxy and resistant to staining with aqueous based stains such as the Gram stain. The primary stain, carbolfuchsin, is …

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Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation It is also know as Ziehl-Neelsen stain. Objective of Acid Fast Stain The main aim objective of this stain is to differentiate bacteria into acid fast group and non-acid fast groups. Principle of Acid Fast Stain Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells waxy and resistant to staining …

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Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation Objectives of Bile Esculin Test This test is used for the presumptive identification of enterococci and organisms in the Streptococcus bovis group. The test differentiates enterococci and group D streptococci from non–group D viridans streptococci. Principle of Bile Esculin Test Gram-positive bacteria other than some streptococci and enterococci are inhibited by the bile salts …

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