Cultural characteristics of Clostridium perfringens

Cultural characteristics of Clostridium perfringens

Cultural characteristics of Clostridium perfringens It is an anaerobic bacteria but can grow under micro-aerophilic condition. They form large, translucent, flat and filamentous colonies with irregular edges. PH: 5.5 to 8.0 (Average PH: 7.2) Temperature: 20°C to 50°C (Average: 37°C) At 45°C, generation time is 10 minutes. Clostridium perfringens on Robertson’s Cooked Meat Broth Saccharolytic … Read more Cultural characteristics of Clostridium perfringens

Habitat and Morphology of Staphylococcus aureus

Habitat and Morphology of Staphylococcus aureus

Habitat and Morphology of Staphylococcus aureus Habitat Natural habitat is mammalian body surfaces. They are normal flora of skin and mucous membrane. Also present in nose / the anterior nares. They can also be found in Pharynx. Found in stratified epithelial cells or mucous or serum constituents associated with these cells. Found in skin/nasal passage … Read more Habitat and Morphology of Staphylococcus aureus

Habitat of Clostridium perfringens

Habitat of Clostridium perfringens

Habitat of Clostridium perfringens Clostridium perfringens are isolated from fecal specimens from human and animals. They are an inhabitant of human normal intestinal flora. They are present in nature and can be found as a normal component of decaying vegetation, marine sediments and soil. Beef, poultry, gravies and dried or pre-cooked foods are common sources … Read more Habitat of Clostridium perfringens

Habitat and Morphology of Mycobacterium tuberculosis

Habitat and Morphology of Mycobacterium tuberculosis

Habitat and Morphology of Mycobacterium tuberculosis Habitat Human is only the know reservoir. Usually found in the lungs but can also live in any part of the body. They may be obligate pathogen, facultative or opportunistic pathogens or free living. Usually infect the mono-nuclear phagocytes. Can also be infected by the consumption of unpasteurized milk. … Read more Habitat and Morphology of Mycobacterium tuberculosis

Growth at 42°C Test- Principle, Procedure and Result Interpretation

Growth at 42°C Test- Principle, Procedure and Result Interpretation

Growth at 42°C Test- Principle, Procedure and Result Interpretation Purpose of Growth at 42°C Test This test is used to differentiate a pyocyanogenic pseudomonads from other Pseudomonas sp. Principle of Growth at 42°C Test The test is used to determine the ability of an organism to grow at 42°C. Several Pseudomonas species have been isolated in the … Read more Growth at 42°C Test- Principle, Procedure and Result Interpretation

Gelatin Hydrolysis Test- Principle, Procedure and Result Interpretation

Gelatin Hydrolysis Test- Principle, Procedure and Result Interpretation

Gelatin Hydrolysis Test- Principle, Procedure and Result Interpretation Objective of Gelatin Hydrolysis Test The production of gelatinases capable of hydrolyzing gelatin is used as a presumptive test for the identification of various organisms, including Staphylococcus sp., Enterobacteriaceae, and some gram-positive bacilli. Principle of Gelatin Hydrolysis Test This test is used to determine the ability of an organism … Read more Gelatin Hydrolysis Test- Principle, Procedure and Result Interpretation

Flagella Stain- Principle, Procedure and Result Interpretation

Flagella Stain- Principle, Procedure and Result Interpretation

Objective of Flagella Stain This technique is used to visualize the presence and arrangement of flagella for the presumptive identification of motile bacterial species. Principle of Flagella Stain Flagella are too thin to be visualized using a bright field microscope with ordinary stains, such as the Gram stain, or a simple stain. A wet mount … Read more Flagella Stain- Principle, Procedure and Result Interpretation

Esculin Hydrolysis Test- Principle, Procedure, Results

Esculin Hydrolysis Test- Principle, Procedure and Result Interpretation

Objective of Esculin Hydrolysis Test This test is used for the presumptive identification and differentiation of Enterobacteriaceae. Principle of Esculin Hydrolysis Test This test is used to determine whether an organism is able to hydrolyze the glycoside esculin. Esculin is hydrolyzed to esculetin, which reacts with Fe3+ and forms a dark brown to black precipitate. Media: … Read more Esculin Hydrolysis Test- Principle, Procedure, Results

DNase Test Agar- Principle, Procedure and Result Interpretation

DNase Test Agar- Principle, Procedure and Result Interpretation

DNase Test Agar- Principle, Procedure and Result Interpretation Objectives of DNase Test Agar (DNA Hydrolysis) This test is used to differentiate organisms based on the production of deoxyribonuclease. It is used to distinguish Serratia spp (positive) from Enterobacter spp., Staphylococcus aureus (positive) from other species, and Moraxella catarrhalis (positive) from Neisseria sp. Principle of DNase Test Agar (DNA … Read more DNase Test Agar- Principle, Procedure and Result Interpretation

Decarboxylase Test- Principle, Procedure and Result Interpretation

Decarboxylase Test- Principle, Procedure and Result Interpretation

Decarboxylase Test- Principle, Procedure and Result Interpretation Objective of Decarboxylase Test This test is used to differentiate decarboxylase producing Enterobacteriaceae from other gram-negative rods. Principle of Decarboxylase Test This test measures the enzymatic ability (decarboxylase) of an organism to decarboxylate (or hydrolyze) an amino acid to form an amine. Decarboxylation, or hydrolysis, of the amino acid results … Read more Decarboxylase Test- Principle, Procedure and Result Interpretation