Flagella Stain- Principle, Procedure and Result Interpretation

Flagella Stain- Principle, Procedure and Result Interpretation

Flagella Stain- Principle, Procedure and Result Interpretation Objective of Flagella Stain This technique is used to visualize the presence and arrangement of flagella for the presumptive identification of motile bacterial species. Principle of Flagella Stain Flagella are too thin to be visualized using a bright field microscope with ordinary stains, such as the Gram stain, … Read moreFlagella Stain- Principle, Procedure and Result Interpretation

Esculin Hydrolysis Test- Principle, Procedure and Result Interpretation

Esculin Hydrolysis Test- Principle, Procedure and Result Interpretation

Esculin Hydrolysis Test- Principle, Procedure and Result Interpretation Objective of Esculin Hydrolysis Test This test is used for the presumptive identification and differentiation of Enterobacteriaceae. Principle of Esculin Hydrolysis Test This test is used to determine whether an organism is able to hydrolyze the glycoside esculin. Esculin is hydrolyzed to esculetin, which reacts with Fe3+ and forms … Read moreEsculin Hydrolysis Test- Principle, Procedure and Result Interpretation

DNase Test Agar- Principle, Procedure and Result Interpretation

DNase Test Agar- Principle, Procedure and Result Interpretation

DNase Test Agar- Principle, Procedure and Result Interpretation Objectives of DNase Test Agar (DNA Hydrolysis) This test is used to differentiate organisms based on the production of deoxyribonuclease. It is used to distinguish Serratia spp (positive) from Enterobacter spp., Staphylococcus aureus (positive) from other species, and Moraxella catarrhalis (positive) from Neisseria sp. Principle of DNase Test Agar (DNA … Read moreDNase Test Agar- Principle, Procedure and Result Interpretation

Decarboxylase Test- Principle, Procedure and Result Interpretation

Decarboxylase Test- Principle, Procedure and Result Interpretation

Decarboxylase Test- Principle, Procedure and Result Interpretation Objective of Decarboxylase Test This test is used to differentiate decarboxylase producing Enterobacteriaceae from other gram-negative rods. Principle of Decarboxylase Test This test measures the enzymatic ability (decarboxylase) of an organism to decarboxylate (or hydrolyze) an amino acid to form an amine. Decarboxylation, or hydrolysis, of the amino acid results … Read moreDecarboxylase Test- Principle, Procedure and Result Interpretation

Coagulase Test- Principle, Procedure and Result Interpretation

Coagulase Test- Principle, Procedure and Result Interpretation

Coagulase Test- Principle, Procedure and Result Interpretation Purpose of Coagulase Test The test is used to differentiate Staphylococcus aureus (positive) from coagulase-negative staphylococci (negative). Principle of Coagulase Test S. aureus produces two forms of coagulase, bound and free. Bound coagulase, or “clumping factor,” is bound to the bacterial cell wall and reacts directly with fibrinogen. This … Read moreCoagulase Test- Principle, Procedure and Result Interpretation

Spore Stain- Principle, Procedure and Result Interpretation

Spore Stain- Principle, Procedure and Result Interpretation

Spore Stain- Principle, Procedure and Result Interpretation Members of the anaerobic genera Clostridium and Desulfotomaculum and the aerobic genus Bacillus are examples of organisms that have the capacity to exist either as metabolically active vegetative cells or as highly resistant, metabolically inactive cell types called spores. When environmental conditions become unfavorable for continuing vegetative cellular … Read moreSpore Stain- Principle, Procedure and Result Interpretation

Capsule Stain- Principle, Procedure and Result Interpretation

Capsule Stain- Principle, Procedure and Result Interpretation A capsule is a gelatinous outer layer that is secreted by the cell and that surrounds and adheres to the cell wall. It is not common to all organisms. Cells that have a heavy capsule are generally virulent and capable of producing disease, since the structure protects bacteria … Read moreCapsule Stain- Principle, Procedure and Result Interpretation

Negative Staining- Principle, Procedure and Result Interpretation

Negative Staining- Principle, Procedure and Result Interpretation

Negative Staining- Principle, Procedure and Result Interpretation Objectives of Negative Staining To perform a negative staining procedure. To understand the benefit obtained from visualizing unstained microorganisms. Principle of Negative Staining Negative staining requires the use of an acidic stain such as India ink or nigrosin. The acidic stain, with its negatively charged chromogen, will not … Read moreNegative Staining- Principle, Procedure and Result Interpretation

Simple Staining- Principle, Procedure and Result Interpretation

Simple Staining- Principle, Procedure and Result Interpretation

Simple Staining- Principle, Procedure and Result Interpretation Objectives of Simple Staining To perform a simple staining procedure. To compare the morphological shapes and arrangements of bacterial cells. Principle of Simple Staining In simple staining, the bacterial smear is stained with a single reagent, which produces a distinctive contrast between the organism and its background. Basic stains with … Read moreSimple Staining- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation Objective Differential staining of nucleic acids including DNA and RNA. Principle Acridine orange, a vital stain, will intercalate with nucleic acid, changing the dye’s optical characteristics so that it will fluoresce bright orange under ultraviolet light. All nucleic acid–containing cells will fluoresce orange. Acridine orange is a … Read moreAcridine Orange Stain- Principle, Procedure and Result Interpretation