Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation Objective of Kinyoun-Cold Method Identification of acid-fast Mycobacterium spp. and parasites such as Cryptosporidium and Isopora spp. Principle of Kinyoun-Cold Method Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells waxy and resistant to staining with aqueous based stains such as the Gram stain. … Read moreAcid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation It is also know as Ziehl-Neelsen stain. Objective of Acid Fast Stain The main aim objective of this stain is to differentiate bacteria into acid fast group and non-acid fast groups. Principle of Acid Fast Stain Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells … Read moreAcid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Gram Stain- Principle, Reagents, Procedure and Result Interpretation

Gram Stain- Principle, Reagents, Procedure and Result Interpretation

Gram Stain- Principle, Reagents, Procedure and Result Interpretation The Gram stain was developed by Christian Gram in 1884 and modified by Hucker in 1921. Objective of Gram Stain This test differentiate the bacteria into Gram Positive and Gram Negative Bacteria, which helps in the classification and differentiation of microorganisms. The Gram stain separates bacteria into two … Read moreGram Stain- Principle, Reagents, Procedure and Result Interpretation

Citrate Utilization Test- Principle, Procedure and Result Interpretation

Citrate Utilization Test- Principle, Procedure and Result Interpretation

Citrate Utilization Test- Principle, Procedure and Result Interpretation Objectives of Citrate Utilization Test The purpose of this test is to identify organisms capable of using sodium citrate as the sole carbon source and inorganic ammonium salts as the sole nitrogen source. The test is part of a series referred to as IMViC (indole, methyl red, Voges-Proskauer, … Read moreCitrate Utilization Test- Principle, Procedure and Result Interpretation

Cetrimide Agar Test- Principle, Procedure and Result Interpretation

Cetrimide Agar Test- Principle, Procedure and Result Interpretation

Cetrimide Agar Test- Principle, Procedure and Result Interpretation Objective of Cetrimide Agar Test This test is primarily used to isolate and purify Pseudomonas aeruginosa from contaminated specimens. Principle of Cetrimide Agar Test The test is used to determine the ability of an organism to grow in the presence of cetrimide, a toxic substance that inhibits the growth … Read moreCetrimide Agar Test- Principle, Procedure and Result Interpretation

Catalase Test- Principle, Procedure and Result Interpretation

Catalase Test- Principle, Procedure and Result Interpretation

Catalase Test- Principle, Procedure and Result Interpretation Objective of Catalase Test This test differentiates catalase-positive micrococcal and staphylococcal species from catalase-negative streptococcal species. Principle of Catalase Test Aerobic and facultative anaerobic organisms produce two toxins during normal metabolism, hydrogen peroxide (H2O2) and superoxide radical (O2−). These bacteria have two enzymes that detoxify the products of normal metabolism. … Read moreCatalase Test- Principle, Procedure and Result Interpretation

CAMP Test- Principle, Procedure and Result Interpretation

CAMP Test- Principle, Procedure and Result Interpretation

CAMP Test- Principle, Procedure and Result Interpretation Objective of CAMP Test The Christie, Atkins, and Munch-Peterson (CAMP) test is used to differentiate group B streptococci (Streptococcus agalactiae–positive) from other streptococcal species. Listeria monocytogenes also produces a positive CAMP reaction. Principle of CAMP Test Certain organisms (including group B streptococci) produce a diffusible extracellular hemolytic protein … Read moreCAMP Test- Principle, Procedure and Result Interpretation

Butyrate Disk Test- Principle, Procedure and Result Interpretation

Butyrate Disk Test- Principle, Procedure and Result Interpretation

Butyrate Disk Test- Principle, Procedure and Result Interpretation Purpose of Butyrate Disk Test This is a rapid test to detect the enzyme butyrate esterase, to aid identification of Moraxella (Branhamella) catarrhalis. Principle of Butyrate Disk Test Organisms capable of producing butyrate esterase hydrolyze bromochlorindolyl butyrate. Hydrolysis of the substrate in the presence of butyrate esterase releases indoxyl, … Read moreButyrate Disk Test- Principle, Procedure and Result Interpretation

Bile Solubility Test- Principle, Procedure and Result Interpretation

Bile Solubility Test- Principle, Procedure and Result Interpretation

Bile Solubility Test- Principle, Procedure and Result Interpretation Objective of Bile Solubility Test This test differentiates Streptococcus pneumoniae (positive–soluble) from alpha-hemolytic streptococci (negative–insoluble). Principle of Bile Solubility Test Bile or a solution of a bile salt (e.g., sodium desoxycholate) rapidly lyses pneumococcal colonies. Lysis depends on the presence of an intracellular autolytic enzyme, amidase. Bile salts lower … Read moreBile Solubility Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation

Bile Esculin Test- Principle, Procedure and Result Interpretation Objectives of Bile Esculin Test This test is used for the presumptive identification of enterococci and organisms in the Streptococcus bovis group. The test differentiates enterococci and group D streptococci from non–group D viridans streptococci. Principle of Bile Esculin Test Gram-positive bacteria other than some streptococci and enterococci are … Read moreBile Esculin Test- Principle, Procedure and Result Interpretation