Bacitracin Susceptibility Test- Principle, Procedure and Result Interpretation

Bacitracin Susceptibility Test- Principle, Procedure and Result Interpretation

Bacitracin Susceptibility Test- Principle, Procedure and Result Interpretation Objectives of Bacitracin Susceptibility Test This test is used for presumptive identification and differentiation of beta-hemolytic group A streptococci (Streptococcus pyogenes– susceptible) from other beta-hemolytic streptococci. It is also used to distinguish staphylococci species (resistant) from micrococci (susceptible). Principle of Bacitracin Susceptibility Test The antibiotic bacitracin inhibits the … Read moreBacitracin Susceptibility Test- Principle, Procedure and Result Interpretation

Acetate Utilization Test- Principle, Procedure and Result Interpretation

Acetate Utilization Test- Principle, Procedure and Result Interpretation

Acetate Utilization Test- Principle, Procedure and Result Interpretation Objective of Acetate Utilization Test To differentiate organisms based on ability to use acetate as the sole source of carbon. Generally used to differentiate Shigella sp. from Escherichia coli. Principle of Acetate Utilization Test This test is used to differentiate an organism capable of using acetate as the sole source … Read moreAcetate Utilization Test- Principle, Procedure and Result Interpretation

Acetamide Utilization Test- Principle, Procedure and Result Interpretation

Acetamide Utilization Test- Principle, Procedure and Expected Results

Acetamide Utilization Test- Principle, Procedure and Result Interpretation Objective of Acetamide Utilization Test To differentiate microorganisms based on the ability to use acetamide as the sole source of carbon. Principle of Acetamide Utilization Test Bacteria capable of growth on this medium produce the enzyme acylamidase, which deaminates acetamide to release ammonia. The production of ammonia results in … Read moreAcetamide Utilization Test- Principle, Procedure and Result Interpretation

Cultural Characteristics of E. coli

Cultural Characteristics of E. coli

Cultural Characteristics of E. coli 1. E. coli is facultative anaerobes. 2. Its optimum growth temperature is 37°C and ranges from 10°C to 40°C. E. coli on Nutrient Agar (NA) 1. They appear large, circular, low convex, grayish, white, moist, smooth and opaque. 2. They are of 2 forms: Smooth (S) form and Rough (R) … Read moreCultural Characteristics of E. coli

Candida albicans- Habitat, Morphology, Cultural Characteristics, Life Cycle, Pathogenesis, Lab Diagnosis, Treatments, Prevention and Control

Candida albicans– Habitat, Morphology, Cultural Characteristics, Life Cycle, Pathogenesis, Lab Diagnosis, Treatments, Prevention and Control Habitat of Candida albicans Normal Habitat: mucosal membranes of human and other warm blooded animals. Also found in the gut, the vagina or also in the surface of the skin. Found in the digestive tract of birds also. Isolated from … Read moreCandida albicans- Habitat, Morphology, Cultural Characteristics, Life Cycle, Pathogenesis, Lab Diagnosis, Treatments, Prevention and Control

Habitat of Bacillus cereus

Habitat of Bacillus cereus

Habitat of Bacillus cereus Bacillus cereus is isolated from soil, vegetables, milk, cereals, spices, fried rice, cooked poultry and meats, soups and desserts. It is also found in mashed potato, beef stew, apples, hot chocolates sold in vending machines and other improper food handling areas. In 1887, B. cereus was isolated from air in a cowshed … Read moreHabitat of Bacillus cereus

Cultural Characteristics of Bacillus cereus

Cultural Characteristics of Bacillus cereus

Cultural Characteristics of Bacillus cereus Most Bacillus spp grow readily on nutrient agar or peptone media. The optimum temperature for growth varies from 20°C to 40°C, mostly 37°C. B. cereus is mesophilic and is capable of adapting to a wide range of environmental conditions. On Nutrient Agar at 37°C, it forms large (2-5 mm) grey-white, … Read moreCultural Characteristics of Bacillus cereus

Morphology of Bacillus cereus

Morphology of Bacillus cereus

Morphology of Bacillus cereus Bacillus cereus is gram positive rod shaped bacilli with square ends. Occasionally may appear gram variable or even gram negative with age. They are single rod shaped or appears in short chains. Clear cut junctions between the members of chains are easily visible. Tissue section staining may appear long and filamentous. … Read moreMorphology of Bacillus cereus

Morphology of Clostridium perfringens

Morphology of Clostridium perfringens

Morphology of Clostridium perfringens Clostridium perfringens is large rectangular gram positive bacilli with rounded or truncated ends. It is pleomorphic with straight or curved rods. Size is about 3-8 µm X 0.4-1.2 µm. It is capsulated, non-motile and non-flagellated pathogenic bacteria. It contains spores with central or sub-terminal spores but spores are rarely seen. Endospores … Read moreMorphology of Clostridium perfringens