Theories of Disease

Theories of Disease

Theories of Disease A disorder of structure or function in a human, animal, or plant, especially one that produces specific signs and symptoms or that affects a specific location and is not simply a direct result of physical injury is called a disease. It is a particular abnormal, pathological condition that affects part or all of … Read more Theories of Disease

Biochemical Test of Pseudomonas aeruginosa

Biochemical Test of Pseudomonas aeruginosa

Biochemical Test of Pseudomonas aeruginosa Basic Characteristics Properties (Pseudomonas aeruginosa) Capsule Non-Capsulated Catalase Positive (+ve) Cetrimide Test Positive (+ve) Citrate Positive (+ve) Coagulase Negative (-ve) Flagella Flagellated (Single) Gas Negative (-ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Negative (-ve) H2S Negative (-ve) Hemolysis Beta Hemolytic Indole Negative (-ve) Motility Motile (Unipolar) MR (Methyl Red) Negative … Read more Biochemical Test of Pseudomonas aeruginosa

Culture media used in Microbiology with their uses

Culture media used in Microbiology with their uses

A7 and A8 agars A7 and A8 agars are selective and differential media used for the cultivation, identification, and differentiation of Ureaplasma spp. and Mycoplasma hominis.   Alkaline peptone water Alkaline peptone water is an enrichment broth used for the isolation of small numbers of Vibrio and Aeromonas organisms from stool specimens.   American Trudeau … Read more Culture media used in Microbiology with their uses

Indole Test- Objective, Principle, Media, Procedure and Results

Result Interpretation of Indole Test

Objective of Indole Test To determine the ability of organism for the production of indole by the action of tryptophanase enzyme.  Principle of Indole Test Tryptophan, an essential amino acid, is oxidized by some bacteria by the action of trytophanase enzyme. Bacteria that produce the enzyme tryptophanase are able to degrade the amino acid tryptophan into … Read more Indole Test- Objective, Principle, Media, Procedure and Results

Characteristic of Genetic Code

Characteristic of Genetic Code

Characteristic of Genetic Code The genetic code is the set of rules by which a linear sequence of nucleotides specifies the linear sequence of a polypeptide. That is, they specify how the nucleotide sequence of an mRNA is translated into the amino acid sequence of a polypeptide. Thus, the relationship between the nucleotide sequence of … Read more Characteristic of Genetic Code

Bird Seed Agar

Result Interpretation of Bird Seed Agar

Bird Seed Agar Bird Seed Agar is a solid medium used for selective and differential isolation of Cryptococcus neoformans from clinical specimens. It is used for the selective isolation of Cryptococcus neoformans and C. gattii. C. neoformans usually grows as a yeast (unicellular) and replicates by budding . Cryptococcus neoformans is an encapsulated yeast that produces the enzyme phenoloxidase, an enzyme necessary … Read more Bird Seed Agar

Laboratory diagnosis, Treatment and Prevention of Mycoplasma pneumoniae

Laboratory diagnosis, Treatment and Prevention of Mycoplasma pneumoniae

Laboratory diagnosis of Mycoplasma pneumoniae Specimen Ideal specimens are throat swabs and nasopharyngeal aspirates, lung biopsies, expectorated sputum. Washings are more reliable than sputum specimens because most infected patients have a dry, nonproductive cough and do not produce sputum. Microscopy Test is not useful because organisms do not have a cell wall and do not … Read more Laboratory diagnosis, Treatment and Prevention of Mycoplasma pneumoniae

Pathogenesis and Clinical Manifestation of Neisseria gonorrhoeae

Pathogenesis and Clinical Manifestation of Neisseria gonorrhoeae

Virulence factors or antigenic structures of Neisseria gonorrhoeae A. Pili Pili are the hair like appendages that extend up to several micrometers from the gonococcal surface. It mediates the exchange of genetic material between strains and attachment to human mucosal cell surface, invasion of host cells, and survival through the inhibition of phagocytosis. Two types: … Read more Pathogenesis and Clinical Manifestation of Neisseria gonorrhoeae

Shiga toxin-producing Escherichia coli (STEC)

Shiga toxin-producing Escherichia coli (STEC)

Shiga toxin-producing Escherichia coli (STEC) The Shiga toxin-producing E. coli (STEC), is also alternatively referred to as verocytotoxin-producing E. coli (VTEC), and enterohemorrhagic E. coli (EHEC). All members of this group are defined by the presence of Shiga toxin 1 (Stx1) or 2 (Stx2). Some but not all EHEC strains are LEE positive and form … Read more Shiga toxin-producing Escherichia coli (STEC)

Habitat and Morphology of Mycobacterium leprae

Habitat and Morphology of Mycobacterium leprae

Habitat of Mycobacterium leprae They are found in soil, water and air. Found in the soils where the leprosy infected people live. Most of them are non-pathogenic and non-parasitic. Found in warm tropical countries. Mycobacterium leprae are found in the peripheral nerves of humans. Found in the cold parts of the body like hand, feet … Read more Habitat and Morphology of Mycobacterium leprae