Biochemical Test of Bordetella pertussis

Biochemical Test of Bordetella pertussis

Biochemical Test of Bordetella pertussis Some of the characteristics are as follows: Basic Characteristics Properties (Bordetella pertussis) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Negative (-ve) Flagella Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram-negative (-ve) Growth in nutrient broth Negative (-ve) Hemolysis Positive (+ve) Motility Negative (-ve) Nitrate Reduction Negative (-ve) Oxidase Positive(+ve) … Read moreBiochemical Test of Bordetella pertussis

Lactobacilli MRS Broth

Lactobacilli MRS Broth

Lactobacilli MRS Broth MRS Broth was developed by deMan, et al., to provide luxuriant growth of lactobacilli isolated from oral, fecal, and other specimen sources. Lactobacilli MRS Broth is an improved medium for lactobacilli, it supports good growth and is particularly useful for a number of fastidious strains which grow poorly on other general media. … Read moreLactobacilli MRS Broth

Digestion and Absorption of Carbohydrates, Proteins and Fats

Digestion and Absorption of Carbohydrates, Proteins and Fats

Digestion and Absorption of Carbohydrates, Proteins and Fats Carbohydrates, fats, and proteins are the major nutrients the body needs for growth, repair, movement and maintaining tissue and organ function. These macromolecules are broken down and absorbed into the body at different rates and into specific forms as they travel through the organs in the digestive … Read moreDigestion and Absorption of Carbohydrates, Proteins and Fats

Cystine Tryptic Agar

Cystine Tryptic Agar

Cystine Tryptic Agar Cystine tryptic agar (CTA), also known as cystine trypticase agar, was developed by Vera as a semi-solid growth medium used for the identification and maintenance of microorganisms for extended periods when stored at appropriate temperatures. It is used for the detection of bacterial motility and, with added carbohydrate, it contributes to fermentation … Read moreCystine Tryptic Agar

Counter Current Immunoelectrophoresis

Counter Current Immunoelectrophoresis

Counter Current Immunoelectrophoresis Immunoelectrophoresis refers to the process in which an antigen mixture is first separated into its component parts by electrophoresis and then tested by immunodiffusion. The electrophoresed antigen mixture in agarose gel allows the separation of different antigens along the gel slide, and then the lateral diffusion of an antibody in the gel … Read moreCounter Current Immunoelectrophoresis

Cystine Glucose Blood Agar

Francisella tularensis on Cystine Glucose Blood Agar

Cystine Glucose Blood Agar Cystine Glucose Blood Agar is known as Cystine Heart Agar. Francis developed blood-dextrose-cystine agar after determining F. tularensis would only grow on an artificial medium supplemented with sulfhydryl compounds (i.e., cystine). F. tularensis is a fastidious organism and requires cysteine for best growth. To recognize the lifetime achievements of Francis in understanding … Read moreCystine Glucose Blood Agar

Biochemical Test of Lactobacillus spp.

Biochemical Test of Lactobacillus spp

Biochemical Test of Lactobacillus spp. Image Source: Creative Market and India Mart. Some of the characteristics are as follows: Basic Characteristics Properties (Lactobacillus spp.) Capsule Negative (-ve) Catalase Negative (-ve) Citrate Negative (-ve) Flagella If present, peritrichous Gas Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram positive H2S Negative (-ve) Indole Negative (-ve) Motility … Read moreBiochemical Test of Lactobacillus spp.

Potassium hydroxide (KOH) test

Potassium hydroxide (KOH) test

Potassium hydroxide (KOH) test Objective To visualize the fungal element in the clinical specimens. To examine skin scrapings or flakes and hair for the presence of hyphae and arthroconidia in suspected dermatophyte infections. Principle Potassium hydroxide (KOH) can be used on clinical specimens to clear cellular material and for better visualization of fungal elements. KOH … Read morePotassium hydroxide (KOH) test

Ouchterlony Double Immunodiffusion technique

Ouchterlony Double Immunodiffusion technique

Ouchterlony Double Immunodiffusion technique Immuno-diffusion is a technique for the detection or measurement of antibodies and antigens by their precipitation which involves diffusion through a substance such as agar or gel agarose. Simply, it denotes precipitation in gel. It refers to one of the several techniques for obtaining a precipitate between an antibody and its … Read moreOuchterlony Double Immunodiffusion technique

Enteroinvasive E. coli (EIEC)

Enteroinvasive E. coli (EIEC)

Enteroinvasive E. coli (EIEC) Enteroinvasive E. coli (EIEC) strains are rare in both developed and developing countries. EIEC infections are characterized by a period of watery diarrhea that precedes the onset of scanty dysenteric stools containing blood and mucus. Pathogenic strains are primarily associated with a few restricted O serotypes: O124, O143, and O164. EIEC … Read moreEnteroinvasive E. coli (EIEC)