Western blot

Synonyms: Immunoblot

Western blot definition

The detection of specific proteins by separating them via electrophoresis, transferring them to a membrane, and adding specific antibodies. (Brock Biology of Microorganisms)

A procedure for separating and identifying antigen or antibody mixtures by two-dimensional electrophoresis in polyacrylamide gel, followed by immune labeling. (Foundation in Microbiology by Talaro and Chess)

A technique that uses antibodies to detect the presence of specific proteins separated by electrophoresis. (Microbiology: An Introduction by Tortora, Funke, and Case)