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Last Updated on June 28, 2020 by Sagar Aryal
Cultural characteristics of Staphylococcus aureus
- Staphylococci grow readily on most bacteriologic media under aerobic or microaerophilic conditions.
- Colonies on solid media are round, smooth, raised, and glistening.
- S. aureus usually forms gray to deep golden yellow colonies.
- Mannitol Salt Agar: circular, 2–3 mm in diameter, with a smooth, shiny surface; colonies appear opaque and are often pigmented, golden yellow.
- Tryptic Soy Agar: circular, convex and entire margin.
- Blood Agar: beta-hemolysis.
- Brain heart infusion agar: Yellow pigmented colonies.
Biochemical characteristics of Staphylococcus aureus
- Catalase positive
- Oxidase negative
- OF test – fermentative
- Coagulase positive: presence of free and /or bound coagulase
- Indole negative
- Gas negative
- Hydrogen sulphide negative
- Methyl red positive
- VP positive
- Nitrate reduction positive
- Gelatin hydrolysis positive
- Beta hemolysis on Blood agar
- Citrate positive
- Motility negative
- PYR negative
- Urease positive