Cultural and Biochemical characteristics of Staphylococcus aureus 4.83/5 (78)


Cultural characteristics of Staphylococcus aureus

  1. Staphylococci grow readily on most bacteriologic media under aerobic or microaerophilic conditions.
  2. Colonies on solid media are round, smooth, raised, and glistening.
  3. S. aureus usually forms gray to deep golden yellow colonies.
  4. Mannitol Salt Agar: circular, 2–3 mm in diameter, with a smooth, shiny surface; colonies appear opaque and are often pigmented, golden yellow. Staphylococcus aureus in Mannitol Salt Agar

  5. Tryptic Soy Agar: circular, convex and entire margin. Staphylococcus aureus on Tryptic Soy Agar
  6. Blood Agar: beta-hemolysis.Staphylococcus aureus colonies on blood agar, beta-hemolysis
  7. Brain heart infusion agar: Yellow pigmented colonies.

Biochemical characteristics of Staphylococcus aureus

  • Catalase positive
  • Oxidase negative
  • OF test – fermentative
  • Coagulase positive: presence of free and /or bound coagulase
  • Indole negative
  • Gas negative
  • Hydrogen sulphide negative
  • Methyl red positive
  • VP positive
  • Nitrate reduction positive
  • Gelatin hydrolysis positive
  • Beta hemolysis on Blood agar
  • Citrate positive
  • Motility negative
  • PYR negative
  • Urease positive

Cultural and Biochemical characteristics of Staphylococcus aureus

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3 comments

  1. I am Hafsat Abdulazeez from Nigeria.I am also doing my master’s degree in Medical Microbiology currently.its nice meeting you here!

  2. if culture staphylococcus on blood Agar but it does not has hemolysis on blood Agar .pleae excuse me is it right or wrong in these culture

  3. excuse me i have one about culture
    if culture staphylococcus on blood Agar but it does not has hemolysis on blood Agar .pleaese excuse me, is it right or wrong in these culture?
    I am sorry if I have mistake about ask the question

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