Answer: Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios. TCBS agar is composed of yeast extract, peptone, sodium thiosulfate, sodium citrate, ox bile (oxgall), sucrose, sodium chloride, iron (III) citrate, bromothymol blue, thymol blue, agar, and distilled or deionized water. TCBS is not autoclaved because: This … Read moreWhy TCBS agar is not autoclaved?
Answer: It is a non-selective, non-differential medium. This means that almost all organisms plated on here will grow. It contains starch. Starch is known to absorb toxins released from bacteria so that they cannot interfere with the antibiotics. It also mediates the rate of diffusion of the antibiotics through the agar. It is a loose … Read moreWhy MHA is used for antibiotic susceptibility testing?