Protocol: Phenol-chloroform extraction of prokaryotic DNA

Protocol Phenol-chloroform extraction of prokaryotic DNA

Protocol: Phenol-chloroform extraction of prokaryotic DNA Deoxyribonucleic acid (DNA) extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. The simplest cells, such as bacteria cells, are prokaryotes. These prokaryotes comprise a lipid bilayer outer membrane and a cytoplasm containing … Read moreProtocol: Phenol-chloroform extraction of prokaryotic DNA

Rapid Method for Enumeration of E. coli Biotype I

Rapid Method for Enumeration of E. coli Biotype I

This method is modified by Anderson and Parker. Prepare serial tenfold dilutions in peptone water of food. Aseptically transfer sterile cellulose acetate membranes to the surface or dried glutamate agar. Transfer duplicate 1.0 mL aliquots of each dilution to cellulose acetate membranes, using a sterile glass Drigalski spreader, distribute the fluid over the entire membrane … Read moreRapid Method for Enumeration of E. coli Biotype I