Cultural Characteristics of E. coli

Cultural Characteristics of E. coli

Cultural Characteristics of E. coli 1. E. coli is a facultative anaerobes. 2. Its optimum growth temperature is 37°C and ranges from 10°C to 40°C. E. coli on Nutrient Agar (NA) 1. They appear large, circular, low convex, grayish, white, moist, smooth and opaque. 2. They are of 2 forms: Smooth (S) form and Rough (R) form. 3. Smooth forms …

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E. coli on m-Endo Agar

E. coli growing on m-Endo Agar with golden-green metallic sheen

– E. coli growing on m-Endo Agar with golden-green metallic sheen. – Photo By: Nurul Azira MJ from Penang, Malaysia. – M-Endo Agar is used for enumeration of coliforms in water using a two step membrane filter method. – Red to red-black colonies with a metallic sheen.

Salmonella Typhimurium on XLD agar

Salmonella typhimurium on XLD agar

Salmonella Typhimurium on XLD agar. Photo By: Carlos Rafael Manzano Palafox from Guadalajara Jalisco, México. – Xylose-Lysine Deoxycholate Agar (XLD Agar) is a selective medium recommended for the isolation and enumeration of Salmonella Typhi and other Salmonella species. – Red colonies, some with black centers. – The agar itself will turn red due to the presence of Salmonella type colonies.

Staphylococcus aureus on combined Mannitol Salt Agar and Vogel & Johnson Agar

Staphylococcus aureus on combined Mannitol Salt Agar and Vogel & Johnson Agar

– Staphylococcus aureus on combined Mannitol Salt Agar and Vogel & Johnson Agar. – Photo By: Luis Alberto Pascual Santiago. – It is used for the selective isolation and differentiation of Staphylococcus aureus from clinical samples. – It forms yellow colonies with yellow zones. – Culture Media is still pink because this is combined media of Mannitol Salt Agar and Vogel …

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Staphylococcus aureus on Columbia CNA Agar with 5% Sheep Blood

Staphylococcus aureus on Columbia CNA Agar with 5% Sheep Blood

– Staphylococcus aureus on Columbia CNA Agar with 5% Sheep Blood. – Photo By: Marlon Neris from Brasília, Brazil. – Columbia CNA Agar is recommended for use as a selective growth medium for the isolation and differentiation of gram-positive cocci from clinical and non-clinical specimens which contain mixed flora. – Large White to Cream Colonies – May be beta-hemolytic

Vibrio cholerae (Owaga) on TCBS Agar

Vibrio cholerae (Owaga) on TCBS Agar

– Vibrio cholerae (Owaga) on TCBS Agar. – Photo By: Q.F.B. Carlos Rafael Manzano Palafox from México. – TCBS Agar is used for the isolation of Vibrio cholerae and other enteropathologic Vibrio (in particular Vibrio parahaemolyticus) in fish, seafood and biological samples of animal origin. – Flat yellow colonies, 2-3 mm in diameter