Cultural Characteristics of E. coli

Cultural Characteristics of E. coli

Cultural Characteristics of E. coli 1. E. coli is a facultative anaerobes. 2. Its optimum growth temperature is 37°C and ranges from 10°C to 40°C. E. coli on Nutrient Agar (NA) 1. They appear large, circular, low convex, grayish, white, moist, smooth and opaque. 2. They are of 2 forms: Smooth (S) form and Rough (R) form. 3. Smooth forms …

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E. coli on m-Endo Agar

E. coli growing on m-Endo Agar with golden-green metallic sheen

– E. coli growing on m-Endo Agar with golden-green metallic sheen. – Photo By: Nurul Azira MJ from Penang, Malaysia. – M-Endo Agar is used for enumeration of coliforms in water using a two step membrane filter method. – Red to red-black colonies with a metallic sheen.

Salmonella Typhimurium on XLD agar

Salmonella typhimurium on XLD agar

Salmonella Typhimurium on XLD agar. Photo By: Carlos Rafael Manzano Palafox from Guadalajara Jalisco, México. – Xylose-Lysine Deoxycholate Agar (XLD Agar) is a selective medium recommended for the isolation and enumeration of Salmonella Typhi and other Salmonella species. – Red colonies, some with black centers. – The agar itself will turn red due to the presence of Salmonella type colonies.

Staphylococcus aureus on combined Mannitol Salt Agar and Vogel and Johnson Agar

Staphylococcus aureus on combined Mannitol Salt Agar and Vogel & Johnson Agar

– Staphylococcus aureus on combined Mannitol Salt Agar and Vogel & Johnson Agar. – Photo By: Luis Alberto Pascual Santiago. – It is used for the selective isolation and differentiation of Staphylococcus aureus from clinical samples. – It forms yellow colonies with yellow zones. – Culture Media is still pink because this is combined media of Mannitol Salt Agar and Vogel …

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Staphylococcus aureus on Columbia CNA Agar with 5% Sheep Blood

Staphylococcus aureus on Columbia CNA Agar with 5% Sheep Blood

– Staphylococcus aureus on Columbia CNA Agar with 5% Sheep Blood. – Photo By: Marlon Neris from Brasília, Brazil. – Columbia CNA Agar is recommended for use as a selective growth medium for the isolation and differentiation of gram-positive cocci from clinical and non-clinical specimens which contain mixed flora. – Large White to Cream Colonies – May be beta-hemolytic

Vibrio cholerae (Owaga) on TCBS Agar

Vibrio cholerae (Owaga) on TCBS Agar

– Vibrio cholerae (Owaga) on TCBS Agar. – Photo By: Q.F.B. Carlos Rafael Manzano Palafox from México. – TCBS Agar is used for the isolation of Vibrio cholerae and other enteropathologic Vibrio (in particular Vibrio parahaemolyticus) in fish, seafood and biological samples of animal origin. – Flat yellow colonies, 2-3 mm in diameter