Biochemical Test of Proteus mirabilis

Biochemical Test of Proteus mirabilis

Biochemical Test of Proteus mirabilis ­Basic Characteristics Properties ( Proteus mirabilis ) Capsule Negative (-ve) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Positive (+ve) Gas from Glucose Positive (+ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Negative (-ve) Growth in KCN Positive (+ve) H2S Positive (+ve) Indole Negative (-ve) Motility Positive (+ve) MR (Methyl Red) Positive (+ve) Nitrate Reduction Positive (+ve) OF (Oxidative-Fermentative) Facultative anaerobes Oxidase Negative (-ve) Pigment Negative (-ve) Shape Rods Spore Negative (-ve) Urease Positive (+ve) VP (Voges … Read more

Biochemical Test of Streptococcus pneumoniae

Biochemical Test of Streptococcus pneumoniae

Biochemical Test of Streptococcus pneumoniae Basic Characteristics Properties (Streptococcus pneumoniae) Bile Solubility Positive (+ve) Catalase Negative (-ve) Gram Staining Positive (+ve) Hemolysis Alfa Hemolysis Motility Non-motile OF (Oxidative-Fermentative) Facultative anaerobes Oxidase Negative (-ve) Shape Diplococci Spore Non-sporing Urease Negative (-ve) VP (Voges Proskauer) Negative (-ve) Fermentation of Arabinose Positive (+ve) Arbutin Negative (-ve) Dulcitol Negative (-ve) Erythritol Positive (+ve) Fructose Positive (+ve) Galactose Positive (+ve) Glucose Positive (+ve) Glycerol Positive (+ve) Glycogen Positive (+ve) Hippurate Negative (-ve) Inulin Positive (+ve) … Read more

Biochemical Test of Bacteria

Biochemical Test of Bacteria

Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are necessary for the bacterium’s individual metabolism, and some facilitate the bacterium’s ability to compete with other bacteria or establish an infection. Tests that measure single bacterial enzymes are simple, … Read more

Biochemical Test of Fusobacterium necrophorum

Biochemical Test of Fusobacterium necrophorum

Image Source: Microbe-Canvas Some of the characteristics are as follows: Basic Characteristics Properties (Fusobacterium necrophorum) Bile Growth Variable Capsule Negative (-ve) Catalase Negative (-ve) Flagella Negative (-ve) Gas Positive (+ve) Gelatin Hydrolysis Variable Gram Staining Gram-negative bacteria H2S Positive (+ve) Hemolysis Positive (+ve) (alpha- and beta-hemolysis may be observed) Indole Positive (+ve) Methylene Blue Reduction Positive (+ve) Motility Negative (-ve) MR (Methyl Red) Negative (-ve) Nitrate Reduction Negative (-ve) Oxidase Negative (-ve) Shape Pleomorphic rod ranging from small, almost coccoid … Read more

Biochemical Test of Aeromonas hydrophila

Biochemical Test of Aeromonas hydrophila

Image Source: Wikipedia Some of the characteristics are as follows: Basic Characteristics Properties (Aeromonas hydrophila) Capsule -ve Catalase +ve Citrate +ve Flagella +ve Gas +ve Gelatin Hydrolysis +ve Gram Staining Gram-negative H2S +ve Hemolysis +ve Indole +ve Maximum Growth Temperature 41°C Motility +ve Nitrate Reduction +ve OF (Oxidative-Fermentative) Fermentative Oxidase +ve Oxygen Requirement Facultative Anaerobe Shape Rod Spore -ve String Test -ve TSIA (Triple Sugar Iron Agar) Alkali/Acid Urease -ve VP (Voges Proskauer) +ve Fermentation of Arabinose Variable Arabitol -ve … Read more

Biochemical Test of Haemophilus aegyptius

Biochemical Test of Haemophilus aegyptius

Some of the characteristics are as follows: Basic Characteristics Properties (Haemophilus aegyptius) Capsule Negative (-ve) Catalase Positive (+ve) Gas Negative (-ve) Gram Staining .Gram-negative H2S Negative (-ve) Hemolysis Negative (-ve) Indole Negative (-ve) Motility Negative (-ve) Nitrate Reduction Positive (+ve) Nitrite Reduction Negative (-ve) Oxidase Positive (+ve) Shape Long slender rods Urease Positive (+ve) V-factor requirement Positive (+ve) Fermentation of Adonitol Negative (-ve) Arabinose Negative (-ve) Cellobiose Negative (-ve) Dulcitol Negative (-ve) Erythritol Negative (-ve) Fructose Negative (-ve) Glucose Positive … Read more

Biochemical Test of Burkholderia cepacia

Biochemical Test of Burkholderia cepacia

a: Burkholderia cepacia ATCC (Image Source: ATCC) b: Burkholderia cepacia (Image Source: Rapid Microbiology) c: Burkholderia cepacia (Image Source: OMICS International) d: Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis (Image Source: Thermo Scientific) e: Scanning Electron Micrograph of Burkholderia cepacia (Image Source: Wikipedia) f: Burkholderia cepacia gram stain (Image Source: DOI: 10.1099/jmmcr.0.005081) Some of the characteristics are as follows: Basic Characteristics Properties (Burkholderia cepacia) Bacteriocin Positive (+ve) Capsule Positive (+ve) Catalase Positive (+ve) Citrate Positive (+ve) … Read more

Biochemical Test of Klebsiella granulomatis

Biochemical Test of Klebsiella granulomatis

Image: Granuloma inguinale (donovanosis) in a conventional Pap test. A: Granulomatous inflammation is shown with epithelioid histiocytes and lymphocytes. B: The organisms (Donovan bodies) can be seen in thin-walled intracytoplasmic vacuoles (circle ). (Pap stain, Mag ×200 in a and ×400 in b). C: Macrophages show numerous safety pin-shaped structures with polar thickening of chromatin (Donovan bodies) and a halo around them (circle) (Pap, ×100). Image Source: PathologyOutlines.com Some of the characteristics are as follows: Basic Characteristics Properties (Klebsiella granulomatis) … Read more

Biochemical Test of Haemophilus parainfluenzae

Biochemical Test of Haemophilus parainfluenzae

Some of the characteristics are as follows: Basic Characteristics Properties (Haemophilus parainfluenzae) Capsule Variable Catalase Variable Gas Variable Gram Staining Gram-negative (-ve) H2S Positive (+ve) Hemolysis Negative (-ve) Indole Variable Motility Negative (-ve) Nitrate Reduction Positive (+ve) Nitrite Reduction Positive (+ve) Oxidase Positive (+ve) Shape Small, pleomorphic rods, usually with long, filamentous forms. Urease Variable V-factor requirement Positive (+ve) Fermentation of Adonitol Negative (-ve) Arabinose Negative (-ve) Cellobiose Negative (-ve) Dulcitol Negative (-ve) Erythritol Negative (-ve) Fructose Positive (+ve) Galactose … Read more

Biochemical Test of Francisella tularensis subsp. tularensis

Biochemical Test of Francisella tularensis

Image Source: New York State Health Department Some of the characteristics are as follows: Basic Characteristics Properties (Francisella tularensis subsp. tularensis) Capsule Positive (+ve) Catalase Positive (+ve) Flagella Negative (-ve) Gas Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Faintly stained Gram-negative H2S (in cysteine-supplemented medium) Positive (+ve) Indole Negative (-ve) Motility Negative (-ve) Nitrate Reduction Negative (-ve) NH3 produced in liquid media Negative (-ve) Oxidase Negative Shape Short, rod-shaped or coccoid Spore Negative (-ve) Sodium ricinoleate solubility Positive (+ve) … Read more