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Prashant Dahal

Prashant Dahal completed his bachelor’s degree (B.Sc.) Microbiology from Sunsari Technical College, affiliated with Tribhuvan University. He is interested in topics related to Antimicrobial resistance, the mechanism of resistance development, Infectious diseases (Pneumonia, tuberculosis, HIV, malaria, dengue), Host-pathogen interaction, Actinomycetes, fungal metabolites, and phytochemicals as novel sources of antimicrobials and Vaccines.

Modified Hodge Test (MHT)- Principle, Procedure, Results, Uses

September 28, 2022September 22, 2022 by Prashant Dahal
Modified Hodge Test (MHT)

Modified Hodge Test (MHT) is a phenotypic method for detecting carbapenemase production by isolated pathogens in clinical laboratories. It is a reliable method to confirm that the carbapenem resistance in … Read more

RT-PCR: Definition, Principle, Enzymes, Types, Steps, Uses

September 25, 2022September 21, 2022 by Prashant Dahal
One-step vs. Two-step RT-PCR

Polymerase chain reaction (PCR) is a temperature-dependent nucleic acid amplification technique used to amplify the DNA or RNA in vitro enzymatically. Developed by Kary Mullis and his associates at mid … Read more

Streak Plate Method- Principle, Types, Methods, Uses

September 20, 2022August 28, 2022 by Prashant Dahal
Streak Plate Method

Streak literally means “a long, thin line”: and the streak plate method is a microbiological culture technique where a sample is spread in a petri dish in the form of … Read more

Pour Plate Method- Definition, Principle, Procedure, Uses

September 20, 2022August 27, 2022 by Prashant Dahal
Pour Plate Method

The pour Plate Method technique was established in the laboratory of Robert Koch and is still being used widely since his period. This method is suitable for facultative, Microaerophilic, and … Read more

Spread Plate Method- Definition, Principle, Procedure, Uses

January 9, 2023August 26, 2022 by Prashant Dahal
Spread Plate Method

Spread Plate Method is one of the widely used culture techniques in microbiology laboratories due to its ease and simplicity. This method is suitable for aerobic and facultative aerobic microorganisms. … Read more

IMViC Test- Principle, Result Chart, Examples, Uses

September 7, 2022July 10, 2022 by Prashant Dahal
IMViC Tests

IMViC test is a series of four different biochemical tests used in identifying and differentiating bacteria, especially the members of Enterobacteriaceae. Though it can be (and is) used for the … Read more

Coliform Bacteria- Characteristics, Examples, Identification

June 9, 2022May 25, 2022 by Prashant Dahal
Coliform Bacteria

Coliform bacteria are a group of Gram-negative, aerobic, and facultative anaerobic rod-shaped bacteria containing the enzyme – galactosidase that is capable of fermenting lactose-producing acid and gas. They are also … Read more

Enterobacteriaceae- Definition, Characteristics, Identification

May 27, 2022May 20, 2022 by Prashant Dahal
Enterobacteriaceae

Enterobacteriaceae is a family of Gram-negative rod-shaped (bacilli) bacteria in the order Enterobacterales of the class Gammaproteobacteria in the phylum Pseudomonadota. They are commonly found in the intestine of animals, … Read more

Eye and Ear Microbiota (Microbiome)- Normal Flora of Eye & Ear

May 21, 2022May 18, 2022 by Prashant Dahal
Normal Flora of Eyes and Ears

Normal floras are the microorganisms that live on the body surface or mucous membrane of a healthy individual without causing any infection. Normal flora includes bacteria and fungi that colonize … Read more

Normal Flora of Skin, Hair, and Nail- Human Skin Microbiome

May 21, 2022May 18, 2022 by Prashant Dahal
Normal Flora of Skin, Hair, and Nail

Normal floras are the microorganisms that live in our body in a relatively stable manner without causing any negative effect on the host’s body. These bacteria and fungi colonize in … Read more

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